retrogenix cell microarray (Charles River Laboratories)

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Charles River Laboratories retrogenix cell microarray

BNT351 shows comparable target affinity to 1-18 and no off-target binding (A) Left: the binding affinity of BNT351 and 1-18 to the stabilized HIV-1 envelope protein trimer (SOSIP BG505.664 ) by SPR (mean and individual values of triplicate measurements). Also see A. Right: binding of BNT351 and 1-18 to SOSIP BG505.664 by ELISA (mean ± standard deviation of triplicate measurements). (B) BNT351’s off-target binding was assessed by Retrogenix Cell <t>Microarray</t> Technology platform. Interactions identified during the library screen with ∼6,500 human proteins are shown. Also, see . Interaction with pepsinogen C (PGC) that appeared to be specific was re-tested by flow cytometry with HEK293 cells transfected with PGC. HEK293 cells transfected with FcγRIIIa and FcεRIg were used as a positive control. Duplicate measurements are shown for flow cytometry.

Retrogenix Cell Microarray, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Microarrays/cell+microarray+screen+technology/pmc13276592-382-1-1
Average 86 stars, based on 1 article reviews

retrogenix cell microarray - by Bioz Stars, 2026-09

86/100 stars

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1) Product Images from "Preclinical assessment of broadly neutralizing HIV-1 antibody BNT351 with optimized pharmacokinetics and potent antiviral activity"

Article Title: Preclinical assessment of broadly neutralizing HIV-1 antibody BNT351 with optimized pharmacokinetics and potent antiviral activity

Journal: iScience

doi: 10.1016/j.isci.2026.116022

Figure Legend Snippet: BNT351 shows comparable target affinity to 1-18 and no off-target binding (A) Left: the binding affinity of BNT351 and 1-18 to the stabilized HIV-1 envelope protein trimer (SOSIP BG505.664 ) by SPR (mean and individual values of triplicate measurements). Also see A. Right: binding of BNT351 and 1-18 to SOSIP BG505.664 by ELISA (mean ± standard deviation of triplicate measurements). (B) BNT351’s off-target binding was assessed by Retrogenix Cell Microarray Technology platform. Interactions identified during the library screen with ∼6,500 human proteins are shown. Also, see . Interaction with pepsinogen C (PGC) that appeared to be specific was re-tested by flow cytometry with HEK293 cells transfected with PGC. HEK293 cells transfected with FcγRIIIa and FcεRIg were used as a positive control. Duplicate measurements are shown for flow cytometry.

Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation, Microarray, Flow Cytometry, Transfection, Positive Control

Expressing:

Article Title: The Bispecific Tumor Antigen-Conditional 4–1BB x 5T4 Agonist, ALG.APV-527, Mediates Strong T-Cell Activation and Potent Antitumor Activity in Preclinical Studies
Article Snippet: .. 5T4 expression was detected using immunohistochemical detection of formalin-fixed, paraffinembedded (FFPE) human tumor microarrays and human and cynomolgus normal tissue microarrays at Charles River laboratories in Frederick, MD, USA. .. Commercially available α-5T4 antibodies (mouse α-5T4, 524731, R&D Systems, 5 g/ml) or rabbit α-5T4 antibody (EPR5529, Abcam, 0.25 g/ml) or negative control antibodies were applied to FFPE sections of positive control cells (CT26-5T4 high, medium, and low expressing), 5T4-negative control cells (CT26.WT) and selected normal human tissues (liver and placenta).

Article Title: Chimeric DNA-RNA hammerhead ribozyme targeting PDGF A-chain mRNA specifically inhibits neointima formation in rat carotid artery after balloon injury.
Article Snippet: .. Vascular injury and treatment with ribozyme microarrays of oligonucleotides that are used as genespecific hybridization targets to quantitatively expression Male Wistar rats (Charles River Breeding Laboratories, of the corresponding genes [13]. ..

Immunohistochemical staining:

Formalin-fixed Paraffin-Embedded:

Microarray:

Article Title: Local control of hepatic phenotype with growth factor-encoded surfaces
Article Snippet: .. Protein arrays were kept in a refrigerator overnight prior to cell cultivation. fig ft0 fig mode=article f1 Fig 1 caption a4 caption a8 Outline of microarray fabrication Cultivation of primary hepatocytes on GF microarrays Adult female Lewis rats weighing 125–200g were purchased from Charles River Laboratories (Boston, MA) and fed with a commercial diet and water. ..

Membrane:

Article Title: Human cell surface-AAV interactomes identify LRP6 as blood-brain barrier transcytosis receptor and immune cytokine IL3 as AAV9 binder
Article Snippet: .. To address this challenge, here we adapt Retrogenix cell microarrays , of the human membrane proteome and secretome to screen natural and engineered AAV capsid interactions with host cells. ..

Article Title: Human cell surface-AAV interactomes identify LRP6 as blood-brain barrier transcytosis receptor and immune cytokine IL3 as AAV9 binder
Article Snippet: .. To screen AAV-binding proteins, we used Retrogenix cell microarrays , of the human membrane proteome and secretome, in which DNA oligonucleotides (oligos) encoding human membrane and secreted proteins are affixed at known slide locations (Fig. ). ..

Bioprocessing:

Article Title: Human cell surface-AAV interactomes identify LRP6 as blood-brain barrier transcytosis receptor and immune cytokine IL3 as AAV9 binder
Article Snippet: .. To screen AAV-binding proteins, we used Retrogenix cell microarrays , of the human membrane proteome and secretome, in which DNA oligonucleotides (oligos) encoding human membrane and secreted proteins are affixed at known slide locations (Fig. ). ..

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